Diagnostic and strain identfication of Canine Parvovirus in Argentina by molecular biology tools

GALLO CALDERON, MARINA; KELLER, L.; IGLESIAS. M.; MALIRAT V; PERIOLO, O; MATTION, N; LA TORRE, J. L.

Puerto Madryn

Congreso; XLVI Reunion Anula de la Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular; 2010

SAIB - Sociedad Argentina de Investigación en Bioquimica y Biologia Molecular

The continuous emergence of new strains of canine parvovirus (CPV), poorly protected by current vaccination, is a concern among breeders, veterinarians, and dog owners around the world. Therefore, the understanding of the genetic variation in emerging CPV strains is crucial for the design of disease control strategies, including vaccines. In this paper, we obtained the sequences of the fulllength gene encoding for the main capsid protein (VP2) of 11 canine parvovirus type 2 (CPV-2) Argentine representative field strains, selected from a total of 75 positive samples studied in our laboratory in the last 9 years. A comparative sequence analysis was performed on 9 CPV-2c, one CPV-2a, and one CPV-2b Argentine strains with respect to international strains reported in the GenBank database. In agreement with previous reports, a high degree of identity was found among CPV-2c Argentine strains (99.6–100% and 99.7–100% at nucleotide and amino acid levels, respectively). However, the appearance of a new substitution in the 440 position (T440A) in four CPV-2c Argentine strains obtained after the year 2009 gives support to the variability observed for this position located within the VP2, three-fold spike. This is the first report on the genetic characterization of the full-length VP2 gene of emerging CPV strains in South America and shows that all the Argentine CPV-2c isolates cluster together with European and North American CPV-2c strains.