PERSONAL DE APOYO
MANZUR Maria jimena
artículos
Título:
Production of recombinant enzymes of wide use for research
Autor/es:
MANZUR MJ, MUÑOZ RV, LUCERO AA, JURI AYUB M, ALVAREZ SE, CIUFFO GM.
Revista:
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
Referencias:
Lugar: Valparaiso-Chile; Año: 2006 vol. 9 p. 291 - 296
ISSN:
0717-3458
Resumen:
For biotechnological purposes, protein expression refers
to the directed synthesis of large amounts of desired
proteins. The aim of the present work was to produce
reverse transcriptase Moloney murine Leukaemia Virus
retro-transcriptase and Taq DNA polymerase, as
bioactive products. In the present paper, we report the
preparation of recombinant enzymes, expressed in E.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
bioactive products. In the present paper, we report the
preparation of recombinant enzymes, expressed in E.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
bioactive products. In the present paper, we report the
preparation of recombinant enzymes, expressed in E.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
Taq DNA polymerase, as
bioactive products. In the present paper, we report the
preparation of recombinant enzymes, expressed in E.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
E.
coli strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
strains. The enzymes produced exhibited quite good
activity, compared with commercial enzymes, allowing
us to replace the last ones for several lab applications.
We are reporting changes and modifications to
standard protocols described. The standard protocols
were modified, i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.
i.e. for the purification step of Taq, a
temperature dependent procedure was designed. The
enzymes produced were used in different applications,
such as PCR, RT-PCR, PCR Multiplex and RAPDs
molecular markers.