INVESTIGADORES
LIJAVETZKY diego Claudio
artículos
Título:
Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance
Autor/es:
CARRARI, F; BENECH-ARNOLD, R; OSUNA-FERNANDEZ, R; HOPP, E; SANCHEZ, R; IUSEM, N; LIJAVETZKY, D
Revista:
GENOME
Referencias:
Año: 2003 vol. 46 p. 253 - 258
ISSN:
0831-2796
Resumen:
The plant vp1 gene encodes a transcription factor originally
identified in maize that participates in the control of the transition
from embryogenesis to seed germination. Different lines of evidence
suggest that vp1 participates in preharvest sprouting (PHS)
resistance in cereals, but the genetic relationship is not yet
established. Thus, the aim of this study was to investigate if there is
a connection between vp1 and the formerly documented quantitative trait loci for PHS in Sorghum bicolor.
This gene was mapped in sorghum using two well-studied inbred lines
displaying contrasting PHS phenotypes. Linkage analysis revealed that
the S. bicolor vp1 (Sbvp1) locus is linked to
markers located on chromosomes 3 and 8 in maize. Analysis indicated
that this gene is not correlated with PHS at a statistically
significant level. Structural analysis of the Sbvp1 gene from
both inbred lines showed a high degree of overall conservation (low
polymorphism), even in the promoter region. These findings, taken
together with the previously observed correlation between the pattern
of expression of this gene and PHS performance, suggest that Sbvp1
might act downstream in the signalling pathway that leads to seed
germination, or even be differentially regulated as a consequence
rather than as a cause of the phenotypic behaviour.