Obtention of fraction from aqueous extracts of L. Divaricata Cav: Production of nitric oxide and hydrogen peroxide on mouse macrophages.

MARTINO, R; LAPADULA, W.J; CASALI, Y.A; DAVICINO, R.C; MATTAR, M.A; MICALIZZI, B.

Mendoza

Congreso; XXVI Reunion Annual de la Sociedad de Biología de Cuyo; 2008

Sociedad de Biología de Cuyo

Larrea divaricata cav. it is known for its inmunomodulatory effects, especially upon the innate immune system. The purpose of this work was to test different effects of fractions from an aqueous extract of L. divaricata “in vitro”. NO and H2O2 production on mouse peritoneal macrophages was determinated. Decoction obtained at 10%w/v from dried leaves was used. The extract was lyophilized. It was re-suspended in chloroform and chromatografied in a column of silica gel using different chloroform/methanol mixes as mobile phase. Five fractions were obtained by evaporation and chromatographied by using thin layer. To treat macrophages, fractions F1, F2, F3 at 25 ìg/ml and 100 ìg/ml were used. The NO production was determined by Griess reaction. A significant decrease (p<0.05) with F1 at both concentrations 25 ìg/ml and 100 ìg/ml was observed. F2 decrease (p<0.05) NO at 25 ìg/ml. The H2O2 synthesis was determinated using a peroxidase inhibitor (sodic azide). A significant increase (p<0.05) with the three fractions at 100 ìg/ml was observed. In conclusion, the three fractions obtained from L. divaricata stimulate the production of reactive oxygen compounds, and could increase the bactericide effects. The F1 and F2 show a possible anti-inflammatory effect, on mouse peritoneal macrophages.