ACTIVITY OF GRAS PRESERVANTS AGAINST Yersinia enterocolitica BIOFILM CULTURED IN BROTH SUPPLEMENTED WITH MEAT JUICE

IRIARTE, HEBE J.; DI MARCO, NATALIA I. ; COLOCHO, FLORENCIA; FAVIER, GABRIELA; MAIER, MARTIN; PUNGITORE, CARLOS; LUCERO ESTRADA, CECILIA

Mendoza

Congreso; XL REUNIÓN ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2022

Sociedad de Biología de Cuyo

ACTIVITY OF GRAS PRESERVANTS AGAINST Yersinia enterocolitica BIOFILM CULTUREDIN BROTH SUPPLEMENTED WITH MEAT JUICEIriarte HJ1,2, Di Marco NI2,3,Colocho F2, Favier GI1, Maier M4, Pungitore C3,5, Lucero Estrada CSM1,21Área de Microbiología e Inmunología de la UNSL, Argentina. 2Instituto Multidisciplinario de Investigaciones Biológicas (IMIBIO -CONICET). 3Área de Química Orgánica, UNSL. 4Instituto de Química Orgánica Eberhard Karls, Universidad de Tubingen, Alemania.5Instituto de Investigación en Tecnología Química (INTEQUI-CONICET-SL). E-mail: hjiriarte@email.unsl.edu.arYersinia enterocolitica is one of the five leading causes of bacterial gastroenteritis in the world. It is transmitted through contaminated foodor water. Pigs are the main reservoir but it is also found in other animals. The biofilm formed on surfaces in direct or indirect contact withfood is considered one of the main factors of cross-contamination in food processing. However, the in vitro study is very different fromthe natural environment conditions, being able to show different growth and/or survival behavior compared to those evaluated in thelaboratory. Currently, the investigation of biofilms in a real meat processing environment is difficult due to technical challenges. Therefore,waste meat fluids (meat juice, MJ) are used to recreate such environment. According to the Food and Drug Administration (FDA), theacronym GRAS defines substances that are used as food additives generally recognized as safe. The objectives of this work were (i) tostudy the effect of different GRAS preservants on Y. enterocolitica biofilms in nutrient broth added with 50% MJ (NB:MJ), and (ii) tocompare values of biofilm inhibition/eradication to minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)of planktonic cells in the same medium. Y. enterocoltica CLC001 bio/serotype 1A/O:7,8-8-8,19 and Y. enterocolitica WAP+ 1B/O:8 werescreened against six GRAS: ascorbic acid (AA), citric acid (CA), lactic acid (LA), phosphoric acid (PA), acetic acid (AcA) and NaNO2.The only GRAS without bactericidal activity was NaNO2. For both tested strains, higher GRAS concentrations were needed to inhibitbiofilm formation, except for AA and PA against Y. enterocolitica CLC001, than planktonic cells; among four to more than 250 foldswere needed for biofilm eradication, and it was not possible to eradicate it with NaNO2, AA and CA. Moreover, PA was also ineffectiveto eradicate the Y. enterocolitica CLC001 biofilm. It was more difficult to inhibit the biofilm formation of Y. enterocolitica WAP+ than Y.enterocoltica CLC001; furthermore, between AcA and LA which were able to eradicate biofilms of both strains, double concentrationswere needed against Y. enterocolitica WAP+ compared with Y. enterocoltica CLC001. It is possible to conclude that the presence of MJin NB favors Y. enterocolitica resistance to food-preservants agents. Furthermore, with this simulation of meat manufacturing processes itwas demonstrated than some compounds able to inhibit the biofilm formation are not capable of eradicate it