THREE TOPICAL PROTEIN DELIVERY SYSTEMS BASED ON POLY(N-VINYLCAPROLACTAM)

ANA S. SONZOGNI; GUY YEALLAND; MRITYUNJOY KAR ; SARAH HEDTRICH ; LUIS GUGLIOTTA; MARCELO CALDERON; VERÓNICA GONZALEZ; ROQUE MINARI

Los Cocos - Córdoba

Simposio; XII Simposio Argentino de Polímeros - SAP 2017; 2017

Topical delivery routes have a lot of advantages over other types of therapeutic agent delivery modalities such oral, intravenous, intramuscular, etc. Some positive features of this application route are the avoidance of first pass metabolism, of gastrointestinal incompatibility, drug level fluctuation, while minimizing undesirable side effect and providing a self-administration suitability [1]. Different designs of topical delivery systems were developed in order to improve their application, such as the cases of hydrogels in macro- and nano-scales (nanogels) [2][3].In this work, the performance of a novel platform for topical protein delivery is compared with macrogels and nanogels, two well-known and studied systems. This platform consists in a nanostructured patch obtained from dispersed hybrid nanogels with film forming ability and thermosensitive properties. The three systems are based in poly(N-vinylcaprolactam), that give them thermosensitive properties, while the hybrid nanogels also contains poly(butyl acrylate) and poly(acrylic acid) to facilitate the patch formation, by particles coalescence, and the skin adhesion. Transition temperature, cytotoxicity, in vitro ovalbumin (as model protein) release-profile and ovalbumin skin penetration were evaluated and compared amongst the three systems. All the formulations resulted thermosensitive, non-cytotoxic and able to load and release ovalbumin. The use of the nanostructured patch in topical delivery studies showed that skin penetration of ovalbumin was higher than that obtained with pure ovalbumin, and with nanogels and macrogels as supports.