Elaboration of levothyroxine-selective molecularly imprinted nanostructured films

FOOKES, FEDERICO; CARLOS BUSATTO; DIANA ESTENOZ; CASIS, NATALIA

San Sebastián

Simposio; XVI Reunión del Grupo Especializado de Polímeros GEP 2022 y XVII Simposio Latinoamericano de Polímeros - SLAP 2022; 2022

GEP-SLAP2022

Molecular imprinting technology is based on theelaboration of a polymeric material with selectivecapacity to adsorb molecules of interest due to thecreation of highly specific sites formed during thesynthesis. [1].Thyroid hormones have numerous vital functionsin the body as they are involved in thedevelopment of the nervous system and regulatevarious metabolic functions. These compoundsare similar in chemical structure to the thyroxine.The hormone levothyroxine (T4) is usually foundin a healthy adult at levels between 100-200 ng/dL[2]. These low concentrations determine thechemical analysis, since they make it difficult tofind efficient methods for detection andquantification.Hydrogels with submicrometer pores have a highsurface-to-volume ratio that gives them lowerresistance to mass transfer. Therefore, thecombination of molecular imprinting technologywith nanostructured films offers the possibility ofdeveloping platforms for rapid detection ofhormones at low concentrations.In the current work, nanostructured MIPs weresynthesized from deposits of SiO2 particles for theselective binding of T4. The morphologicalparameters of the developed materials and theirT4 adsorption capacity were studied.