INVESTIGADORES
ESTENOZ Diana Alejandra
congresos y reuniones científicas
Título:
Molecularly Imprinted Hydrogels from Colloidal Crystals for the Detection of Progesterone
Autor/es:
NATALIA CASÍS; CARLOS BUSATTO; SERGE RAVAINE; MARIA MARTA FIDALGO DE CORTALEZZI; DIANA ESTENOZ
Lugar:
Buenos Aires
Reunión:
Simposio; X Simposio Argentino de Polímeros, SAP 2013; 2013
Institución organizadora:
Univ. Nac. de Buenos Aires
Resumen:
The
synthesis of molecularly imprinted polymers (MIPs) from crystal colloidals is
investigated. The novel procedure combines the non-covalent imprinting method
with the colloidal crystal template technique to produce membranes with
pre-specified morphology able to selectively recognize progesterone. The
colloidal crystals based on silica particles were obtained by Langmuir-Blodgett
and self-assembly techniques, and exhibited a considerable control of the morphology.
Hydrogel films were prepared by copolymerization of acrylic acid and ethylene
glycol dimethacrylate using 2,2´-azobisisobutyronitrile as initiator and in the
presence of progesterone. The polymerization took place in the interspaces of
the colloidal crystal and after the reaction the silica particles were etched
with hydrofluoric acid to produce a 3D-ordered structure and the progesterone
was removed using acetic acid. The nanocavities derived from progesterone, were
distributed within the internal structure of the films. The equilibrium
swelling properties of the MIPs were studied as a function of crosslinking
degree and pH. The pore morphology of the film was analyzed by SEM. The MIPs
characterizations included Fourier Transform Infrared Spectroscopy (FTIR),
Differential Scanning Calorimetry (DSC) and evaluation of sensing properties and
selectivity.