Phenolic composition of folk medicinal plants with dermatological relevance by core-shell reversed phase liquid chromatography- multiple wavelength detection

ESPINO, MAGDALENA; MONASTERIO, ROMINA; GOMEZ, MARÍA ROXANA; SILVA MARÍA F.

Simposio; 21st International Symposium on Electro- and Liquid Phase-Separation Techniques, 20th Latin-American Symposium on Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis (LACE); 2014

Skin is an important organ that protects us from environmental conditions. It is exposed every day to a large number of agents such as oxidative stress, UV radiation, skin pathogens, etc. that could be the cause of many skin diseases: hyperpigmentation, inflammation, melisma among others.Plants have been traditionally used in medicine. Indeed, the possibility of using plant extracts for skin treatments is a promising area of research. Botanical reports on Argentinian folk medicinal plants with pharmacological relevance have been highlighted; yet there is still limited scientific data to support claims for their effectiveness against dermatological pathologies.In this work, a simple methodology using ultrasound-assisted liquid extraction as a clean-up procedure for herbal medicines (Origanum vulgare, Thymus vulgaris, Morus alba, Matricaria recutita, and Larrea divaricata) has been applied. The determination of phenolic compounds in the extracts was carried out by core-shell reversed-phase liquid chromatography with multiple wavelength detection. Phenolic compounds of pharmaceutical interest (syringic acid, gallic acid, tyrosol, catechin, α-hydroxyphenylacetic acid, 2,5-dihydroxybenzoic acid, caffeic acid, p-coumaric acid, sinapic acid, ferulic acid, apigenin, quercetin, vanillic acid, rutin, and cinnamic acid) were successfully separated with a core shell column, Kinetex C18 (100 x 4.6 mm, 2.6 μm) from Phenomenex in less than 20 min at a flow rate of 0.5 mL/min.References:1. Krajewski A, Garg M, Rajiv Y. Chandawarkar RY, J Plastic Reconstruct Aesth Surg 2010, 63, 896, 905.2. Ko H-H, Chiang Y-C, Tsai M-H, Liang C-J, Hsu L-F, Li S-Y, Wang M-C, Yen F-L, Jee C-W. J Ethnopharmacol 2014,151, 386-393.Acknowledgements:The authors would like to gratefully acknowledge the financial support received from ANPCyT and CONICET.