SIMULTANEOUS DETERMINATION OF CLORAMPHENICOL, SALICYLIC ACID AND RESORCINOL IN PHARMACEUTICAL PREPARATIONS BY CAPILLARY ZONE ELECTROPHORESIS

GOMEZ, MARÍA ROXANA; SILVA MARÍA F.; OLSINA, ROBERTO

Simposio; 7mo Simposio Latinoamericano en Aplicaciones de la Electroforesis Capilar y Tecnología del Microchip en Biotecnología, Biomedicina, Biofarmacia e Industria, (LACE 2001); 2001

Acne is a disease of the sebaceous glands that affects 80% of the population between 12 and 25 years old. Astringent alcoholic lotions are generally used for its treatment, antiseptics such as salicylic acid, parabens and cresols are useful against inflamation and seborrhea, while bacteriostatic products such as cloramphenicol, tetracyclines and erythromicine are common active compounds in acne formulations. Quality control for pharamaceutical procucts is a key topic for the pharmaceutical industry. CE and HPLC are widely used in pharmaceutical analysis for impurity determinations, main component assays, chiral separations, trace level residue monitoring and product stability studies, among others. Both techniques can bring methods admitting acceptable validation and routine implementation. Nevertheless, CE is the methodology of choice because it offers significant advantages in terms of operating costs and quicker method development. This study demonstrates the separation of active ingredients in acne formulations (salicylic acid, cloramphenicol and resorcinol in presence of azulene) by capilary zone electrophoresis. Factors affecting their separations were the buffer pH and concentration, applied voltaje, sample preparation, presence of additives Optimun results were obtained with a 50 mM sodium tetraborate, pH 9.22. The carrier electrolyte gave baseline separation with good resolution, short migration times, great reproducibility and accuracy. Calibration plots were linear over at least three orders of magnitude of analyte concentrations. The procedure was fast and reliable and commercial pharmaceuticals could be analised without prior sample clean-up procedure.