Fingerprint Analysis and Synthetic Adulterant Search in Hedera helix Formulations by Capillary Electrophoresis

CIANCHINO, VALERIA; ORTEGA, CLAUDIA; ACOSTA, GIMENA; MARTINEZ, LUIS DANTE; GOMEZ, MARÍA ROXANA

PHARMAZIE

Govi Verlag

Año: 2007 vol. 62 p. 262 - 265

0031-7144

A high-performance capillary electrophoretic (CE) method has been developed for obtaining electropherograms of the different extracts and the commercial formulation (fingerprints) from Hedera helix L used in our country for the cough’s treatment. Also, a capillary zone electrophoresis (CZE) methodology was developed for the search, identification and determination of some possible adulterants. These feasible adulterants are common synthetic drugs used in respiratory diseases (antitussive, decongestant and bronchodilator agents). Under the optimum conditions, the analytes (ephedrine, codeine, diphenhydramine and constituents of H. helix formulations) were separated within less than 10 min in a 20 mM sodium tetraborate buffer (pH 9.0). The present procedure was validated respect to selectivity, linearity range, limits of detection (LOD) and quantification (LOQ), precision (repeatability and intermediate precision), solutions stabilities and accuracy; the results obtained were satisfactory. Good linearity was obtained over two orders of magnitude and detection limits (S/N=3) were better than 1.2 mg ml–1 for all analytes. The CE methodology was successfully applied to the search and the subsequent determination of ephedrine, codeine and diphenhydramine in the Hedera helix extracts and its phytopharmaceutical products.