Analysis of Metal Profile in Soybean (Glycine max L.) after Cadmiun-induced Oxidative Damage

FELICI, EMILIANO; ALMEYDA, CESAR; FERNANDEZ BALDO, MARTIN; MARTINEZ, LUIS DANTE; ZIRULNIK, FANNY; GOMEZ, MARÍA ROXANA

Journal of Coastal Life Medicine

Haikou Yongfa Printing Co.

Lugar: Haikou; Año: 2014 vol. 2 p. 471 - 477

Objective: To analyze the effect of cadmium (Cd) on soybean seedlings growth and the relationship with the distribution and concentration of macro-microelements. Methods: The ions concentrations were determined by ICP-MS. The extraction efficiency and digestion time were optimized. Also, oxidative stress parameters were determined and related with metal content. Results: The accumulated amount of dry matter in roots and leaves was lower in the Cd-treated group. Regression analysis showed that the exposure to Cd affected the accumulated amount of dry matter as well as the content of mineral elements in the analysis samples. In Cd treated plants, electrical conductivity increased respect to the controls, indicating that ionic permeability became altered. A strong inhibition of the chlorophylls (chl) biosynthesis in the Cd-treated group was also demonstrated by a decrease of chla and chlb concentration. This result was related with the observed significant decreased in the Mg uptake at the roots and leaves level. Conclusions: The stress caused by Cd exposure, evidenced by significantly high hydrogen peroxide levels in root and leaves after 24 h and the content of specific macro-microelements is a factor that affects the accumulation of dry matter, electrical conductivity and dry matter, electrical conductivity and chlorophylls concentrations.