Production of 5-hydroxymethyl-2-furan carboxylic acid by Serratia marcescens from crude 5-hydroxymethylfurfural

MUÑOZ, TATIANA; RACHE, LEIDY Y.; ROJAS, HUGO A.; ROMANELLI, GUSTAVO P.; MARTINEZ, JOSÉ J.; LUQUE, RAFAEL

BIOCHEMICAL ENGINEERING JOURNAL

ELSEVIER SCIENCE SA

Año: 2020 vol. 154

1369-703X

A valuable monomer in the synthesis of various polyesters is 5-hydroxymethyl-2-furan carboxylic acid (HMFCA).In this work, the synthesis of HMFCA using crude HMF obtained from fructose is described. To obtain crudeHMF, fructose dehydration was performed by thermal treatment with Nb 2 O 5 , obtaining a yield of 76%. Then, thecrude HMF was evaluated in the production of HMFCA using Serratia marcescens at different conditions such asinitial optical density (OD), pH, temperature, and substrate concentration. Once the conditions were optimized,the HMFCA concentration of 790.2mg/L was obtained using the fed-batch strategy.