Immobilization of the Lipase B from Candida antarctica on Urban Solid Waste

CARLOS R. LLERENA SUSTER; CYNTHIA FUENTES; JORGE E. SAMBETH; CARLA JOSE

Catalysts

MDPI

Lugar: Basilea; Año: 2023 vol. 13 p. 1324 - 1350

: The adsorption of the lipase B from Candida antarctica (CALB) over polyethylene terephthalate (PET), polypropylene (PP), and derivatives, abundant components of urban solid waste (USW),was investigated. The characterization of the supports and biocatalysts synthesized by SEM-EDS andFTIR is presented. Two immobilization strategies were evaluated, conventional and total adsorption.The adsorbed protein was determined by Bradford and through high-resolution inductively coupledplasma atomic emission spectroscopy (ICP-AES). In this sense, the adsorption of CALB in all theproposed supports was evidenced, obtaining the highest protein loads in bis-(2-hydroxyethyl) terephthalate (BHET). Subsequently, the biocatalysts were applied to the esterification of rac-ibuprofen withethanol. CALB immobilized in BHET showed remarkable activity, achieving conversions of 30%. Inthis context, immobilization on this support was optimized, studying the addition of sorbitol-glycerol.Thus, in the presence of 0.91 g of polyols, a catalyst with a protein load of 33.3 mg·g−1 was obtained,achieving productivity of 0.298 mmol min−1 mg−1. Additionally, no differences were found whenusing BHET from USW bottles of various colors. This research shows the potential of materialsderived from PET as enzymatic supports, unreported materials, that we can use as tools to achievesustainable biotechnological applications.