The complete genomic sequence of an in vivo low replicating BLV strain

DUBE SYAMALIMA; ABBOT LYNN; DUBE DIPAK; DOLCINI GUILLERMINA; GUTIERREZ SILVINA; CERIANI CAROLINA; JULIARENA MARCELA; FERRER JORGE; PERZOVA RAISA; POIESZ BERNARD

VIROLOGY JOURNAL

BIOMED CENTRAL LTD

Año: 2009 vol. 3 p. 120 - 129

1743-422X

DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain afterinoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate,low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplifiedwith a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLVARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequencesincluding an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina.Characterization of BLV ARG 41´s deduced proteins and its relationship to other members of thePTLV/BLV genus of retroviruses are discussed.