DEVELOPMENT OF AN IMMUNOBIOTIC EVALUATION SYSTEM USING BOVINE MAMMARY EPITHELIAL CELLS

MICHIHIRO TAKAGI; RYOYA KOMATSU; LEONARDO ALBARRACÍN; FLAVIA MANSILLA; AMINUL, MD ISLAM; YOSHIHITO SUDA; WAKAKO IKEDA-OHTSUBO; TOMONORI NOCHI; GRACIELA VIGNOLO; HISASHI ASO; JULIO VILLENA; HARUKI KITAZAWA

Conferencia; LXVII Reunión Científica Anual de la Sociedad Argentina de Inmunología; 2019

Mastitis, the inflammation of mammary glands, is one of the most costly diseases of dairy industry, which is often caused by pathogenic bacteria. Antimicrobials are applied for the treatment and prevention of bovine mastitis but their indiscriminate use arouses concerns of residual contamination and increased risk of emerging drug-resistant bacteria. Immunoregulatory probiotics (immunobiotics) are considered as potential substitute for antimicrobials for the prevention of bovine mastitis. In this work, an in vitro system for the evaluation of immunobiotics with beneficial effects against mastitis was developed by using an originally established bovine mammary epithelial cell line (BMECs). BMECs ability to respond to TLR4 activation was evaluated by stimulating cells with LPS for 12h and analyzing the global gene expression changes with a porcine microarray-based transcriptomic assay. A total of 429 genes were differently expressed after LPS stimulation and the most significant change was the up-regulation of inflammatory cytokines and chemokines (p