Development of an in vitro immunobiotic evaluation system against rotavirus infection in bovine intestinal epitheliocytes

HISAKAZU KOBAYASHI; PAULRAJ KANMANI; TAKAMASA ISHIZUKA; AYAKO MIYAZAKI; JUNICHI SOMA; LEONARDO ALBARRACIN; YOSHIHITO SUDA; TOMONORI NOCHI; HISASHI ASO; NORIYUKI IWABUCHI; JIN-ZHONG XIAO; TADAO SAITO; JULIO VILLENA; HARUKI KITAZAWA

Beneficial Microbes

Wageningen Academic Publishers

Lugar: Wageningen; Año: 2017

1876-2883

The bovine intestinal epithelial cell line (BIE cells) expresses the Toll-like receptor (TLR)-3 and is able to mount an antiviral immune response after the stimulation with poly(I:C). In the present study, we aimed to further characterize the antiviral defense mechanisms in BIE cells by evaluating the innate immune response triggered by rotavirus infection. In addition, we attempted to determine whether immunobiotic bifidobacteria are able to confer protection of BIE cells against rotavirus infection by beneficially modulating the antiviral immune response. Rotavirus OSU (porcine) and UK (bovine) effectively infected BIE cells, while a significant lower capacity to infect BIE cells was observed for human (Wa) and murine (EW) rotavirus. We observed that viral infection in BIE cells triggered TLR3/RIG-I-mediated immune responses with activation of IRF3 and TRAF3, induction of IFN-β and up-regulation of inflammatory cytokines. Our results also demonstrated that preventive treatments with Bifidobacterium infantis MCC12 or B. breve MCC1274 significantly reduced rotavirus titers in infected BIE cells and differentially modulated the innate immune response. Of note, both strains significantly improved the production of the antiviral factor IFN-β in rotavirus-infected BIE cells. In conclusion, this work provides comprehensive information on the antiviral immune response of BIE cells against rotavirus, that can be further studied for the development of strategies aimed to improve antiviral defenses in bovine intestinal epithelial cells. Our results also demonstrate that BIE cells could be used as a newly immunobiotic evaluation system against rotavirus infection for application in the bovine host.