Levels of organochlorines in Odontesthes bonariensis during different reproductive stages in La Florida (San Luis, Argentina)

RODRÍGUEZ, N.; NAVARRO BECERRA, N.; CID, F.D.; ANTÓN, R. I.; ENRIQUE CAVIEDES VIDAL

Mendoza

Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2005

LEVELS OF ORGANOCHLORINES IN Odonthestes bonariensis DURING DIFFERENT REPRODUCTIVE STAGES IN LA FLORIDA (SAN LUIS, ARGENTINA) Rodriguez N, Navarro Becerra N, Cid FD, Anton RI, Caviedes- Vidal E. Lab Biol., Univ Nac San Luis, 5700 San Luis. E-mail nfara @unsl.edu.ar La Florida (33"07´S-66"02´W) is an important water reservoir for the province of San Luis. Environmental pollutants, such as organochlorines (OCs) may affect aquatic wildlife. Recently, we reported the presence in La Florida of OCs in the water and fish. OCs are liposoluble and are accumulated in the fat. Several authors have demonstrated that, during the fish reproductive period, a mobilization and transference of the lipids of the body to eggs and sperm occurs. An interesting problem is whether spawning represents an important route to eliminate OCs. To test this idea we analyzed the OCs concentration in silversides (O. bonariensis) captured in La Florida at different reproductive stages: before, during and after the reproductive period. OCs were assayed by GC-ECD. We detected 16 OCs (ng/g lipid), divided in 4 family: 1) HCHs (A: 1079,7- B:1184,8-D:747,7): a, P, y, 6 HCH isomers; 2) DDTs (A:3387,5- B:2408,3-D:2928,3): DDE, DDD, DDT and Methoxychlor; and cyclodiene groups 3a) CHLs (A:802,8-B:862,3-D:541,0): Chlordanes and Heptachlors; 3b) ALDs: (A: 1207,9-B: 145 1,s- D:880,2). No significant differences among reproductive stages were detected (ANOVA, p > 0,05). This fact may suggest that spawning does not represent an important route of elimination the OCs in the silverside.