Intestinal passive absorption of hidrosoluble compounds in Galea musteloides.

FASULO, V.; ANTÓN, R. I.; CHEDIACK, J.G.; BRAGAGNOLO, L.; KARASOV, W. H.; ENRIQUE CAVIEDES VIDAL

Merlo, San Luis

Congreso; Reunión Anual de la Socidad de Biología de Cuyo; 2004

INTESTINAL PASSIVE ABSORPTION OF HYDROSOLUBLE COMPOUNDS IN Galea niusteloides Fasulo Anton R, Chediack J Bragagnolo L, Karasov WH*, Caviedes- Vidal E Unidadlnvest Cs Biol, Univ Nac Sun Luis, 5700 Sun Luis; *DWLE, Univ Wisconsin, Madison (Wi) USA. E-mail: vfasulo@unsl.edu.ar We have demonstrated that intestinal absorption of glucose by the small intestine in birds occurs mostly through the paracellular pathway .(75-100%). In contrast, studies in mammals report that paracellular glucose absorption is small (15-25%). However, all mammalian data have been obtained in anesthetized animals using pelfusion techniques. In this study we measured intestinal absorption in "intact" animals (G. musteloides) and estimated the contribution of the paracellular (PC) and transcellular (TC) route for nutrients absorption. Non metabolizable sugars were used: 3-0- methyl-D-glucose (30MG) (PM 192) as a marker for both routes (TC+PC) and L-rhamnose (PM 182) and cellobiose (PM 342) for the paracellular pathway. Sugars were administrated orally (VO) and injected intramuscularly (IM) to the animals (n = 5, body mass = 229.33 k 26.9 g). Plasma sugar concentrations were measured in serial blood samples after both VO and IM administration using HPLC/Fluorescence. The amount of sugars absorbed (FA) was calculated from areas under the post-absorption and post-injection plasma curves (AUC,and AUC,,) with a classical pharmacokinetic equation FA = [AUC,]/[AUC,,]. FA was: 68.02 f 16.34,16.30 + 4.61 and 7.92 k1.45 (mean k SEM)f or 30MG, L-rhamnose and cellobiose respectively. In these mammals, the contribution of the paracellular route is smaller than in birds of similar body size.