IG Y INDEX VARIATION THROUGHOUT A FAST-REFEEDING PERIOD IN COMMON PIGEON (Columba livia)

MOLINA MARINO, LUCIANA MARIEL; LOPEZ, LEANDRO ERNESTO; CAVIEDES VIDAL, ENRIQUE

San Francico

Congreso; 2013 Annual meeting Society for Integrative and Comparative Biology; 2013

Society for Integrative and Comparative Biology

Humoral immunity, antibody-mediated, is the aspect of the immune system that stands in defense against extracellular pathogens and toxin neutralization among other functions. The most abundant isotype in blood is IgY. One way to characterize the humoral status of an individual is to evaluate the profile of Ig Y under different conditions. In this study we assessed the IgY index of the common pigeon along a fast-refed trial. Pigeons (N =11) received water ad lib and were fasted until a body mass loss of 30-35 % (day F) of the initial day (day 0). Then, birds were refed until they reached their initial body mass (day RF). On days 0, F and RF plasma samples were obtained. We performed an ELISA using different dilutions (1/2500 to 1/20000) of plasma and anti chicken IgY HRP conjugated antibody (1/250). Dilution of plasma gave the same proportional response for each treatment (P=0.91). The effect of nutritional status on the index IgY resulted in a significant difference (F 2,84 =6.99 P