Development of a more rapid and simple procedure for two-dimensional oligonucleotide analysis of foot-and-mouth disease virus: RNA minifingerprinting

BERGMANN, I.E.; TIRABOSCHI, B.H.; AFFONSO BOLLER, M.A.; MALIRAT, V.; PEREIRA, P.J.V.; AUGÉ DE MELLO, P.

BOLETIN DEL CENTRO PANAMERICANO DE FIEBRE AFTOSA

Año: 1989 vol. 55 p. 35 - 42

0009-0131

The success of immunization programs depends, up to some extent on a precise characterization of the viral strains that circulate in the field and on the evaluation of their similarity with the strains used to prepare the vaccines. At present several biochemical techniques are available like RNA fingerprinting, recombinant DNA and rapid sequencing to study, with significant precision, the genetic characteristics of the viral strains. The two-dimensional analysis of RNase T1 resistant oligonucleotides is adequately fit to evaluate the evolutive paths between closely related viral strains, and in particular, for foot-and-mouth disease virus, it has been successfully applied for diagnosis approaches.