Use of a recombinant foot-and-mouth disease virus 3D polymerase in an agarose gel immunodiffusion test

BERGMANN, I.E; MALIRAT, V; FALCZUCK, A.; SONDAHL, M.

BOLETIN DEL CENTRO PANAMERICANO DE FIEBRE AFTOSA

Año: 1999 vol. 64 p. 35 - 39

0009-0131

In the present study, examination was made of the effectiveness of a complete recombinant 3D polypeptide in an AGID test (AGID-3D), for use in the detection of FMDV-infection-specific antibodies, regardless of vaccination condition. Results indicate that compared with the traditional AGID VIAA, the AGID 3D offers, particularly when assessing low titer sera, a more consistent method, with comparable specificity, and at least equal sensitivity. Neither of the antigens offered any particular advantage with regard to band differentiation. Replacement of the VIAA by a recombinant 3D antigen has considerable attractions, since it provides an unlimited supply of a safe, inexpensive, easily purified and consistent material, eliminating the potential presence of non-specific BHK or capsideal antigens