Dermal Papilla Cells cultured as spheres improve angiogenesis

OPPENHEIMER, FLORENCIA MAIA; PROIETTI, CECILIA JAZMIN; CERUTI, JULIETA MARÍA; HAGELIN KARIN; LEIRÓS, GUSTAVO JOSÉ; BALAÑÁ , MARÍA EUGENIA

EXPERIMENTAL DERMATOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2024

0906-6705

Tissue-engineered skin represents a helpful strategy for the treatment of deep skin injuries. Nevertheless, these skin substitutes must promote and encourage proper vascularization for a successful graft take. Previous work showed that Dermal Papilla Cells favor an earlier neovascularization process of grafted skin substitute contributing to the rapid maturation of the neovascular network, reducing inflammation and favoring extracellular matrix remodeling in nude mice. Based on these results, we studied the influence of DPC and their culture conditions on the different stages of angiogenesis in in vitro models.Here, we showed that DPC cultured as spheres favor the expression of angiogenic factors such as VEGF, FGF2, and angiogenin compared to their monolayer culture. To study the effects of DPC on the different stages of angiogenesis, an in vitro model has been adapted. DPC cultured as spheres significantly enhanced HUVEC migration and tubule formation, indicating the importance of employing physiological culture systems that provide a closer representation of cell behavior and interactions occurring in vivo.Overall, these results allow us to speculate that the use of DPC spheres in skin substitutes could promote its grafting, vascularization, and vascular network maturation through the secretion of angiogenic factors. This approach has great potential to improve clinical outcomes in regenerative medicine and skin wound repair.