Transition metal complexes with 6-methoxyquinoline as antitumor agents. Biological studies on two and three-dimensional cell cultured models

CADAVID VARGAS, J.F.; VILLA PÉREZ C.; RUIZ M.C; LEÓN I.E.; VALENCIA-URIBE G,C; SORIA D.B; DI VIRGILIO, A.L.; ETCHEVERRY, S.B.

Mar del Plata

Congreso; LXI Reunión Anual De La Sociedad Argentina De Investigación Clínica (SAIC); 2016

Thecoordination complexes have been extensively studied as antiproliferativeagents, offering a potential alternative for the cancer treatment, where theligand and the metal combination play an important role in the determination ofthe pharmacological properties.This study evaluates and compares the effect of6-methoxyquinoline complexes with copper, zinc, silver, and cobalt as antitumoral agents in 2D(monolayer) and a 3D (multicellularspheroids) models derived from A549 and MG-63 cell lines.The cell viability was measured by the MTT andacid phosphatase assays for the 2D and 3D models respectively. The reactiveoxygen species production was determined using dihydrorhodamine 123 as afluorescent probe.The spheroids were cultured by the liquidoverlay technique and were treated with the complexes for 48h, morphologicalchanges were followed using phase contrast microscopy and image processingusing the software ImageJ. After the treatment, some spheroids where placed ona new plate with fresh medium and fetal bovine serum for 24h to evaluate thepresence of viable cells capable to attach on the surface.Preliminary studies on synergy of the complexeswere performed using the Median-Drug Effect analysis for the Cu and Zncomplexes at fixed ratio concentrations (1:1, 1:3 and 1:5), the affected fractionwas measured after 24h of incubation with the compounds.The Ag, Cu and Zn complexes significantly reducedthe cell viability (P