Red Blood Cell Senescence in Chagas Disease

STAMPONE, ROCÍO; PACINI, ANTONELLA; ENSINCK, ALEJANDRA; DINATALE BRENDA; PEREZ ANA ROSA; COTORRUELO CARLOS; VILLAR SILVINA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias. SAI.SAI.FAIC.SAFIS; 2022

Sociedad Argentina de Inmunología- Sociedad Argentina de Investigación Clínica- Sociedad Argentina de Fisiología

(159) RED BLOOD CELL SENESCENCE IN CHAGASDISEASERocío Stampone1, Antonella Pacini1, Alejandra Ensinck2,Brenda Dinatale1, Ana Rosa Pérez1, Carlos Cotorruelo1, SilvinaVillar11Instituto de Inmunología Clínica y Experimental Rosario (IDICER-CONICET-UNR), Rosario, Argentina2Área de Inmunología. Facultad de Ciencias Bioquímicas yFarmacéuticas, UNR, Rosario, ArgentinaE-mail: rociostampone@gmail.comRed blood cells (RBC) have a life expectancy of 120 days; however,there is evidence supporting that chronic inflammatory processescan alter RBC lifespan. Chagas disease (CD) is an infectious andinflammatory condition with the possibility of modifying the half-life ofRBC. This study aims to identify possible aging mechanisms of RBCin patients with CD. For this, the relationship between RBC agingand membrane marker changes which lead to the clearance of senescenterythrocytes was investigated. Our study was designed toevaluate young RBC (YRBC) and senescent RBC (SRBC) in individualswithout Chronic Chagas Cardiomyopathy (Indeterminate formIND, n=4) and with Chronic Chagas Cardiomyopathy (CCC, n=4).Healthy volunteers were matched according to sex and age (Co,n=5). Flow cytometry has been applied for detection of RBC boundImmunoglobulin G (IgG), CD47 and Annexin V. RBC were gated onthe basis of their forward and side scatter characteristics (YRBC:FSC high; SRBC: FSC low). The percentage of YRBC decreased inboth IND and CCC vs Co. Also, the percentage of SRBC increasedin both IND and CCC vs Co (*p