LIPOTEICHOIC ACID IS INVOLVED IN THE IMMUNOMODULATORY ACTIVITIES OF Lactobacillus plantarum CRL 1506

HIROYA MIZUNO; LORENA ARCE; MARÍA DANIELA VERA; KAE TOMOTSUNE; MD. AMINUL ISLAM; VIZOSO PINTO, MARÍA GUADALUPE; LEONARDO ALBARRACÍN; HIDEKI TAKAHASHI; HARUKI KITAZAWA; VILLENA, JULIO

Jornada; LVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA; 2019

Studies have demonstrated that lipoteichoic acid (LTA) is involved in the immunomodulatory properties of some immunobiotic lactobacilli. The aim of this work was to evaluate whether LTA contributes to the capacity of Lactobacillus plantarum CRL1506 of modulating the intestinal innate antiviral immune response. A D-alanyl-lipoteichoic acid biosynthesis protein knockout CRL1506 strain (ΔdltDCRL1506) was obtained and its ability to modulate TLR3-mediated immune response was evaluated in vitro in porcine intestinal epithelial (PIE) cells and, in vivo in 6-week-old Balb/c mice. Wild-type CRL1506 (WtCRL1506) was used as positive control. The challenge of PIE cells with the TLR3 agonist poly(I:C) significantly increased IFN-β, IL-6 and MCP-1 expressions. PIE cells pretreated with ΔdltDCRL1506 or WtCRL1506 (108 cells during 48 h) showed higher levels of IFN-β (p