Gamma Carbonic Anhydrase like complex interact with plant mitochondrial complex I.

PERALES M; PARISI G; FORNASARI M; ALEJANDRO COLANERI

PLANT MOLECULAR BIOLOGY

SPRINGER

Lugar: Berlin; Año: 2004

0167-4412

We report the identification by two hybrid screens of two novel similar proteins, called Arabidopsis thaliana gamma carbonic anhydrase like1 and 2 (AtcCAL1 and AtcCAL2), that interact specifically with putative Arabidopsis thaliana gamma Carbonic Anhydrase (AtcCA) proteins in plant mitochondria. The interaction region that was located in the N-terminal 150 amino acids of mature AtcCA and AtcCA like proteins represents a new interaction domain. In vitro experiments indicate that these proteins are imported into mitochondria and are associated with mitochondrial complex I as AtcCAs. All plant species analyzed contain both AtcCA and AtcCAL sequences indicating that these genes were conserved throughout plant evolution. Structural modeling of AtcCAL sequences show a deviation of functionally important active site residues with respect to cCAs but could form active interfaces in the interaction with AtcCAs. We postulate a CA complex tightly associated to plant mitochondrial complex.