Natural plant promoters of biofilms and bioemulsifiers increase healthy bacteria resistance as a new promising strategy for pharmaceutical biotechnology

VERNI C.; GARAY J.; ARAUJO J.; BARDON A.; CARTAGENA E.

Córdoba Capital

Congreso; 3º Reunión Internacional de Ciencias Farmacéuticas-RICiFa 2014-; 2014

Natural plant promoters of biofilms and bioemulsifiers increase healthy bacteria resistance as a new promising strategy for pharmaceutical biotechnologyVerni C.1, Garay J. 1, Araujo J. 1, Bardón A.1,2, Cartagena E.1*1 Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. 2 INQUINOA-CONICET. Ayacucho 471 (4000), San Miguel de Tucumán, Argentina. mariaceciliaverni@gmail.com; ecartagena@fbqf.unt.edu.ar Key words: Natural plant promoters, Health beneficial Lactobacillus, biofilms and bioemulsifiers´ production.Lactobacilli, are well known to have a positive effect on the maintenance of human health. These probiotic bacteria constitute natural microbiota, are recognized as potential interfering bacteria by producing various antimicrobial agents such as organic acids, antimicrobial substances, bacteriocins, and adhesion inhibitors, such as biotensioactives, among others. They have antimicrobial activity and capability to interfere with the pathogens adhesion on epithelial cells of urogenital and intestinal tracts, and for their anti-biofilm production on catheter devices and voice prostheses. Some Lactobacillus strains have biofilm forming ability, and release thermostable glycoproteins as main resistance strategies regulated by quorum-sensing mechanism. The aim of this research was to increase biofilms and biotensioactives´ biosynthesis of L. casei mediated by plant natural products. L. casei ssp. paracasei C2 isolated from regional cheeses was screened for their ability to produce biofilms, and biotensioactives. A dewaxing chloroform extract from Flourensia fiebrigii (Asteraceae), a fraction thereof that containing prenylated flavonoids, 2´,4´-dihydroxychalcone (DC) at 50 µg/ml, ascorbic acid (AA), and a mixture of DC with AA (12.5 µg/ml of each one) were analysed as potential promoters. The natural products were incorporated to bacterial culture and incubated for 24 h at 37 °C. Then, biofilms formation was measured by crystal violet technique (8 replicates).The strongest increase of biofilm formation was observed when medium was supplemented with DC (295%), and also with AA (256%) with respect to control strain (100%). Biotensioactives´ synthesis was also increased because all supernatants (and replicates) obtained displace the oil and spread in the water more than supernatant control (from 136 to 200%). These results would be promissory in pharmaceutical biotechnology to improve Lactobacillus adaptation, and the solubilisation of hydrophobic drugs by environment friendly bioemulsifiers.