INTERACTION BETWEEN THE NUCLEAR FACTOR KAPPA B (NF-KB) AND THE ESTROGEN RECEPTOR ALPHA (ERA) IN THE CELLULAR SENESCENCE PROCESS DURING THE DEVELOPMENT OF ESTROGENINDUCED PITUITARY TUMORS

MONGI BRAGATO B; GRONDONA E; SOSA LDV; CARREÑO L; PETITI JP; GUTIÉRREZ S; TORRES A; DE PAUL AL

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA

Evidences from our group demonstrated the emergence ofcellular senescence process as a growth control mechanism duringthe progression of estrogen-induced pituitary tumors. Also,estrogen exerts a modulatory action on pituitary cells proliferation.Considering the preponderant role of NF-kB (p65) in cellularsenescence and points of convergence between ERa andNF-kB signaling pathways in cell cycle control, we evaluate thecontribution of the interaction between these two proteins in thepituitary senescence in experimental pituitary tumors. Wistar adultmale rats were implanted subcutaneously with silastic capsulescontaining estradiol benzoate (30mg) for 10, 20, 40 and 60 days(E10-60). The control group was implanted with empty capsules.Subsequently, ERa:NF-kB immunoprecipitation was performedat the different stages of tumoral development. NF-kB and IkBalevels were also determined from nuclear and cytosolic fractionsby Western blot. The ERa:NF-kB co-localization was analyzed byimmunofluorescence (IF) and transmission electron microscopy(TEM). Statistical analysis: ANOVA-Fischer test (p