CONTRIBUTION OF DIFFERENT SIGNALING PATHWAYS ON THE DEVELOPMENT OF ESTROGEN-INDUCED PITUITARY TUMORS: THEIR ROLE IN SENESCENCE PROCESS

MONGI BRAGATO B; GRONDONA E; SOSA LDV; CARREÑO L; LUQUES JN; PETITI JP; GUTIÉRREZ S; TORRES A; DE PAUL AL

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE LAS SOCIEDADES DE BIOCIENCIA 2017; 2017

SOCIEDAD DE BIOCIENCIA

Recently we have demonstrated signs of cellular senescence as a growth control mechanism during the progression of experimental pituitary tumors. Also, it is known that Ras induces negative feedback response on PI3K/Akt and ERK1/2 pathways, while JNK and p38-stress signalling activation mediate key molecular events, which overall trigger the cellular senescence program. Based on these evidences, we aimed to evaluate the contribution these signalling pathways on pituitary cellular senescence during the development of estrogen-induced tumors.Pituitary tumors were induced in Wistar adult male rats which were implanted with silastic capsules containing estradiol benzoate (30mg) for 10, 20, 40 and 60 days (E10-60). Control group was implanted with empty capsules. Subsequently, phosphorylated (p) and total ERK1/2; AKT; JNK and p38 protein levels were determined by Western blot. Also, pERK1/2, pAKT and senescent associated -b-Galactosidase (SA-b-Gal) double staining was performed in cryosections from normal and tumoral pituitaries. Statistical analysis: ANOVA-Fischer test (p