OXIDATIVE STRESS MODULATES GSK3β SIGNALING ASSOCIATED WITH LIPOLYSIS IN FAT CELLS

FUNK, MELANIA I.; MANISCALCHI, ATHINA; BENZI JUNCOS, ORIANA N.; ALZA, NATALIA P.; CONDE, MELISA A.; SALVADOR, GABRIELA A.; URANGA, ROMINA M.

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Oxidative stress (OS) modulates fat metabolism and triggers chronicinflammation, promoting the onset of metabolic diseases such astype 2 diabetes and obesity. We have previously demonstrated thatiron-induced OS increases lipolysis in mouse white adipose tissueas well as in in vitro differentiated adipocytes. We found that irontreatment triggered β-catenin upregulation and exacerbated lipolysisby ATGL activation. In addition, adipocytes where β-catenin hadbeen deleted showed no ATGL upregulation by OS. Our aim wasto study the role of GSK3β kinase in the modulation of β-catenincascade in adipocytes exposed to OS. For this purpose, we useddifferentiated 3T3-L1 adipocytes (0.5 mM IBMX, 1 μM dexamethasone,6 μg/ml insulin, and 5 μM rosiglitazone) exposed to 500 μMferric ammonium citrate (FAC) for 24 h. We also analyzed adiposetissue and liver isolated from mice challenged with iron overload.As previously reported in adipose tissue, OS enhanced lipolysis inliver (p