Human neural rosettes secrete extracellular vesicles enriched in neural and glial cellular components

BERTONE AROLFO, MATÍAS; HERRERA LÓPEZ, MALENA; GASTALDI, LAURA; REMEDI, MÓNICA; CESCHIN, DANILO; CÁCERES, ALFREDO; MOYANO, ANA LIS

Congreso; PRIMER ENCUENTRO DEL CLUB DE LA GLÍA CONO SUR. Lo bueno, lo malo, lo lindo y lo feo de las células gliales.; 2022

Club de la Glía

Extracellular vesicles (EVs) participate in neural and glial development in the central nervous system (CNS). Human neural rosettes (hNRs) are radial structures of neuroepithelial cells that assemble during human induced pluripotent stem cell (hiPSCs) differentiation into neural and glial cells. Neural rosettes are an in vitro model that recapitulate some stages of the neural tube morphogenesis and express many of the molecular components expressed in vivo during neural tube formation. Here we showed that hiPSCs and hNRs secrete EVs (hiPSC-EVs and hNR-EVs) enriched in proteins related to EVs, exosomes and components of the endomembrane system. Moreover, hNR-EVs are also associated with neural and glial cellular components involved in CNS development. Through in silico analysis we found that at the cellular level these proteins exhibit differential expression profiles at various time points during development and culture conditions. These results indicate that EVs secreted by hNRs exhibit neural and glial cellular components and might provide an innovative approach to study their biological effects during human neural tube formation.