Quantitative Analysis of Sulfatides in Mouse Plasma Membrane Using UHPLC-MS-MS

LI, GUANNAN; MOYANO, ANA LIS; GIVOGRI, MARIA I; VAN BREEMEN, RICHARD B

Minneapolis

Congreso; 61ST ASMS Conference on Mass Spectrometry and Allied Topics; 2013

American Society for Mass Spectrometry

Sulfatides are galactosyl-3-sulfated-ceramide sphingolipids present in plasma membranes of mammalian brain tissue. Sulfatides, other sphingolipids and cholesterol are enriched in lipids rafts. The distribution of sulfatides in the plasma membrane is under investigation to provide direct evidence for the association of sulfoglycolipids with detergent-resistant membranes. Therefore, we developed and validated a highly sensitive and selective method for the measurements of sulfatides in plasma membranes using UHPLC-MS-MS.