Expression of fibronectin during porcine placentation

VELEZ C; MARTÍN P; HERNANDEZ M; WILLIAMSON D; KONCURAT M

Foz de Iguazu

Simposio; V Latin American Symposium on Maternal Fetal Interaction & Placenta IV Latin American Symposium on Reproductive Immunology; 2013

Porcine placenta is epitheliochorial, diffuse, folded, adecidua, and noninvasive. Fibronectin (FN) plays a role in cell adhesion, migration, differentiation, implantation, embryogenesis and oncogenic transformation.The aim of the studywas to investigate the expression of FN during porcineplacentation. Placental tissue samples from sows on gestation days (gd) 30,60, 70 and 114 and non-pregnant uterus samples were used (n¼18). Thedetection of FN was performed by indirect immunoperoxidase using biotinylatedantibodies and streptavidin-HRP, and was visualized by DAB. Themost intensive staining for FN was found in conceptus trophectoderm,particularly in the apical membrane at 30 gd; the staining was weaker at60 and 70 gd and negative at term of pregnancy. FN was not expressed inglandular and endometrial epithelium in all investigated periods. Theresults of this study suggest that the fetal origin FN could participate in themolecular events leading to successful placentation in pigs.Keywords: Fibronectin, porcine, placentation