Alteration of key metabolic components in a follicular persistence model in dairy cows

GAREIS, N.C.; CATTANEO, L.; DÍAZ, P.U.; ORTEGA, H.H.; SALVETTI, N.R.; HEIN, G.J.; REY, F.

Buenos Aires

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR; 2021

Sociedad Argentina de Investigación Clínica

Several local metabolic factors are involved in the ovarian follicular persistence associated with anovulation similar to the ovaries from cows with cystic ovarian disease. Therefore, we aimed to study the protein expression of AdipoR1, AdipoR2, AMPK, CPT1 and ACOX1 in ovarian follicular structures during the development of follicular persistence in cows. Non-lactating Holstein cows (n=25) with regular estrous cycles without previous reproductive disease were studied. The persistence model was performed with an intravaginal progesterone device to get subluteal concentrations of progesterone, obtaining dominant follicles by ovariectomy at the expected day of ovulation (n=5; P0) and follicles that persist for 5 (n=5; P5), 10 (n=5; P10) or 15 days (n=5; P15) relative to the expected time of ovulation. Controls cows, without progesterone treatment, were ovariectomized in proestrus (n=5; C). Expression of target protein was assessed by indirect immunohistochemistry in ovarian tissue sections. In theca cells, AdipoR1 expression was higher in persistent follicles of P15 than persistent/dominant follicles of other groups (p