Alterations of adiponectin receptors and 5-AMP Kinase expression in folicular structures of cows with Cystic Ovarian DIsease

GAREIS, N.C.; DÍAZ, P.U.; SALVETTI, N.R.; PANZANI C.; ORTEGA, H.H.; HEIN, G.J.

Mar del Plata

Congreso; LXIV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2016

Sociedad Argentina de Investigación Clínica

Adiponectin is an adipokine produced by adipose tissuewhich regulates several reproductive processes. Its role insteroidogenesis, considering the energy balance for the ovaryfunctions, could be potentially associated to the development ofreproductive disorders such as cystic ovarian disease (COD).The aim of this study was to determine the expression of componentsof the adiponectin system in ovarian follicles of cowswith spontaneous COD and control cows. Thus, the receptorsof adiponectin, AdipoR1 and AdipoR2, and the enzyme 5-AMPkinase (AMPK) were evaluated. Animals with spontaneous COD(n=8) and control cows (n=8) were ovariectomized and ovariesobtained were processed histologically to embedded in paraffin.Expression of target protein was assessed by indirect immunohistochemistryon ovarian tissue sections. Results showedsimilar expression of AdipoR1 in follicular structures evaluatedin ovaries from control cows and cows with COD, showing atendency to decrease the expression in granulosa cells of cystsregarding to antral follicles (as reference structure) of controlcows (p