Operant conditioning with a stimulus discrimination: An alternative method for evaluating alcohol reinforcement in preweaning rats

GUTTLEIN, LARISA; MOLINA, JUAN CARLOS; ABATE, PAULA

JOURNAL OF NEUROSCIENCE METHODS

ELSEVIER SCIENCE BV

Año: 2021 vol. 363

0165-0270

Background: Ethanol exposure at early ontogeny promotes further predisposition to consume the drug. Operant conditioning allows motivational alcohol properties to be assessed. To date, the operant conditioning approach used during infancy consisted in paired subjects being trained to learn an operant response, using simultaneously a yoked partner, which received reinforcer solution as a result of a paired animal instrumental response (OYS). New method: In our study, we attempted to evaluate ethanol reinforcing effects during PDs 15?18 in an operant conditioning schedule with a stimulus discrimination procedure (OSD), as an alternative control learning. This new proposal includes a single subject, who has to choose between an S+ nose-poke hole, which delivers the reinforcer into the mouth, or an S- nose-poke hole with no reinforcement effect. Results: The OSD results seemed to be more reliable than those obtained using the OYS procedure, since some data appeared to be more robust when using a yoked nose-poke hole than when employing a yoked subject, such as in control learning. Consequently, OSD has the following advantages compared to the OYS procedure: a) the operant response learned is controlled by the overall behavior of the same subject, resulting in a relatively clearer data; b) a yoked animal is not necessary, thereby reducing the number of rats used in the operant conditioning procedure. Comparison with existing methods and conclusions: A novel technique of operant conditioning adapted to infancy was developed by training animals to emit a particular response to gain access to alcohol solution as a reinforcer.