Dam Early Free Access to Hypertonic NaCl Solution Induces a Long-Term Effect on Offspring Basal Chronic Brain Cell Activity

MACAGNO, AGUSTINA; CINTIA, PORCARI; LAURA, VIVAS; AGUSTIN ANASTASIA; ANDREA GODINO

Carlos Paz

Congreso; XXXIV Reunión Anual SAN 2019; 2019

Sociedad Argentina de Investigación en Neurociencias

Exposure to hyperosmotic environment during a pre/postnatal period can differentially program the fluid intake and excretion pattern in a way that persists until adulthood. Our results suggest that maternal voluntary ingestion of hypertonic NaCl solution during pregnancy and lactation until one week post-weaning (MP-Na) alters the offspring?s central osmoregulatory mechanisms. Our aim was to evaluate the impact of MP-Na on the basal expression of TRPV1 osmosensitive channel (by western blot) and chronic neuronal activity along the brain osmosensor areas of adult?s offspring by the immunohistochemical detection of brain Fra like protein (Fra-LI), alone or combined with vasopressin (AVP). The imprinting animals (MP-Na group) showed increased Fra-Li immunoreactivity(ir) in the organum vasculosum of the lamina terminalis (OVLT)(p=0.018) but not in the subfornical organ. Fra-AVP-ir neurons along the supraoptic nucleus(SON) and in the lateral magnocelular subdivision of the paraventricular nucleus(PaLM) show a significant decreased and increased respectively in MP-Na animals(SON p=0.03 and PaLM p=0.02).The expression of TRPV1 in OVLT and SON were not significantly different after MP. Taking into account our previous evidence, these results indicate that the availability of a rich source of NaCl during the perinatal period induces a long-term effect on drinking and the basal neural activity along the OVLT,SON and PVN nuclei implicated in the control of hydroelectrolyte balance.