PERINATAL PROGRAMMING WITH HYPERTONIC SODIUM: EFFECTS ON SODIUM INTAKE AND HYPERTENSION INDUCED BY DOCA-SAL MODEL

PORCARI, CINTIA Y.; CAEIRO, XIMENA E.; GODINO, ANDREA

BUENOS AIRES

Congreso; REUNION ANUAL SAFIS 2023; 2023

SOCIEDAD ARGETINA DE FISIOLOGIA

Introduction: Our previous results indicate that voluntary maternal intake of hypertonic NaCl during gestation/lactation affects the offspring sodium/water intake, brain mRNA expression of angiotensin receptor 1a(Agtr1a), vasopressin, heteronuclear vasopressin, basal number of renal glomeruli, renal gene expression of the TRPV1 channel and Agtr1a and causes a sustained increase of blood pressure after a sodium overload.Objective: Evaluating the effects of perinatal imprinting induced by voluntary consumption of hypertonic NaCl on: -the pattern of hypertension onset in adult offspring, using the desoxycorticosterone acetate and Salt (DOCA-Salt) hypertensive model; - the 1% NaCl intake; - the body size and brain mRNA expression of Agtr1a, and serotonin receptor 2c(Htr2c).Methods: The study was done with Wistar Rats, where the manipulation period (MP) embraced dams from 1 week before conception until offspring at postnatal day 28.Experimental groups: MP-NaCl: NaCl (0.45M), food, and water; MP-Control: food and water (perinatal treatment). On postnatal day 50, male offspring MP-Control and MP- NaCl were subjected to DOCA treatment for 4 weeks (25 mg/kg twice a week) and water was replaced with a 1% NaCl solution. The systolic blood pressure was evaluated in conscious rats using the non-invasive tail cuff method, intake volume of 1% NaCl, and mRNA expression of Agtr1a, Ht2c receptors in SFO and PVN. Results: the DOCA-salt hypertensive treatment to PM-NaCl and PM-Ctrol animals did not differentially modify blood pressure between them and it did not affect theexpression of Agtr1a and Ht2c receptors, but salt consumption significantly increased in PM-NaCl groups [F (1,10) = 11.907;p=0.006].Conclusion: These results, in addition with previous data, indicate that the availability of a rich source of NaCl solution during the perinatal period induces long-term changes in osmoregulatory mechanisms and circuits, altering the functioning of the renal and brain angiotensin and vasopressin systems, which together modulate behavioral, endocrine, and renal responses to achieve hydroelectrolytic and cardiovascular homeostasis after different challenges (including DOCA-SAL).