INVESTIGADORES
DULBECCO Andrea Belen
artículos
Título:
Deltamethrin Binding to Triatoma infestans (Hemiptera: Reduviidae) Lipoproteins. Analysis by Solvent Bar Microextraction Coupled to Gas Chromatography
Autor/es:
DULBECCO, A. B.; MIJAILOVSKY, S. J.; GIROTTI, J. R.; JUÁREZ, M. P.
Revista:
JOURNAL OF MEDICAL ENTOMOLOGY
Editorial:
ENTOMOLOGICAL SOC AMER
Referencias:
Año: 2015 vol. 52 p. 1254 - 1259
ISSN:
0022-2585
Resumen:
The binding of deltamethrin (DLM) to the hemipteran Triatoma infestans (Klug)hemolymph lipoproteins was evaluated in vitro. After DLM incubation with the insect hemolymph,lipoproteins were fractioned by ultracentrifugation. DLM binding was analyzed by a microextractivetechnique?solvent bar microextraction?a solventless methodology to extract DLM from each lipoproteinfraction. This is a novel use of the technique applied to extract an insecticide from an insect fluid.Capillary gas chromatography with microelectron capture detection was used to detect DLM bound bythe T. infestans hemolymph lipoproteins and to identify the preferred DLM carrier. We show that Lpand VHDLp I lipoproteins are mainly responsible for DLM transport in T. infestans, both in DLM-resistantand DLM-susceptible bugs. Our results also indicate that DLM amounts transported are not relatedtoDLM susceptibility.
