Chronic Lymphocytic Leukemia increase neutrophil survival and promote their differentiation into CD16high CD62Ldim sub-set

GIORDANO, MIRTA; ENRIQUE PODAZA; RISNIK, DENISE; ANA COLADO; ELÍAS, ESTEBAN ENRIQUE; ALMEJÚN, MARÍA BELÉN; FERNÁNDEZ GRECCO, HORACIO; VICENTE, ANGELES; MAHUAD, CAROLINA; AIZPURUA, FLORENCIA; BEZARES, RAIMUNDO FERNANDO; BORGE, MERCEDES; GAMBERALE, ROMINA

Buenos Aires

Congreso; Reunión conjunta de sociedades de biociencia; 2017

Abstract: Chronic lymphocytic leukemia (CLL) is characterizedby the progressive accumulation of clonal B lymphocytes in blood,lymph nodes, spleen and bone marrow. Within lymphoid organs,CLL cells foster a protective microenvironment where they surviveand proliferate. While almost every cell type in lymphoid tissueswas reported to be modified by the leukemic clone, there is scarceinformation regarding the interaction of CLL cells with neutrophils.Tumor associated neutrophils (TANs), described in multiple typesof solid tumors, facilitate cancer progression. Our aim was to determineif CLL cells were capable of modifying neutrophil phenotypeand survival. To that purpose, neutrophils from healthy donor(HD) were co-cultured with purified leukemic cells from CLL patients(ratio 1:1) and apoptosis was assessed by flow cytometry usingAnnexin-V. Our results showed that CLL cells delayed neutrophilapoptosis at 24-72 h (n=15, p