PON-1 and SR-B1 polymorphism association in type 2 Diabetes Mellitus.

L.E. FARIAS ALTAMIRANO; G.V. MENDOZA ; S.E. SIEWERT ; M.S. OJEDA

SAN LUIS

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo.; 2012

High levels of High Density Lipoproteins(HDL)are inversily related to cardiovascular disease(CAD)by its atheroprotective role. In Type 2 Diabetes Mellitus (T2DM)subjects HDL-c levels are decreased. The enzime Paraoxonase-1 (PON-1)has an anti-atherogenic effect and protects lipoproteins from the oxidative stress. PON-1 activity is reduced in T2DM patients. Scavenger Receptor class B Type 1 (SR-B1)participates in reverse cholesterol transport by mediatung the selective uptake of free cholesterol. Several polymorphisms have been studied in PON-1 and SR-B1 genes.Decreased PON-1 activity is associated with rs705379 (-107C/T)polymorphisms. In SR-B1, rs838896(IVS 11, G/C)could be related with decreased receptor expression and HDL-c levels modification.The aim of this study was to determinate the association between these polymorphisms and T2DM.52 DNA samples were analyzed (33 diabetics and 19 controls).Genotypes were identified by Tetra Primer ARMS-PCR, allelic and genotypic frequencies were calculated. Rs705379 genotypic frecuencies in Co and Diab were C/C:84% vs 51.5%,C/T: 15.7% vs 45.4%, T/T: 0% vs 3.03%. In rs838896 were G/G:47.3% vs 24.2%, G/C:47.3% vs C/C:5.2% vs 21.2%. HDL-c levels significantly decreased in diabetics with two or more mutations in these genes (p