STUDY OF THE NUCLEAR RECEPTORS NR4A PARTICIPATION IN THE IMMUNE-ENDOCRINE RESPONSE DURING TUBERCULOSIS

GALLUCCI, GEORGINA; D'ATTILIO LUCIANO; DIAZ, ARIANA; BONGIOVANNI, BETTINA; FERNANDEZ, ROCIO; LIOI, SUSANA; BERTOLA, DIEGO; GARDEÑEZ, WALTER; ARMANDO, MELISA; BAY, MARÍA LUISA; BOTTASSO, OSCAR; SANTUCCI, NATALIA

Buenos Aires

Congreso; Congreso. LXVI Reunión Anual de la SAIC; 2021

Tuberculosis (TB) is a major infectious disease caused by Mycobacterium tuberculosis that infectsalveolar macrophages and hence promotes a cellular immune response, which becomes harmfulwhen prolonged over time. Nuclear receptors (NRs) are factors that may modulate the immuneresponse (IR) and inflammation, with a role in the regulation of homeostasis and bacterialpathogenesis. Within the NRs, NR4As orphan receptors have emerged as important regulators ofimmune cell polarization and NF-κB signaling, which can lead to a switch from acute to chronicinflammatory responses. NR4A receptors modulate NF-κB activity in a dynamic manner, eitherrepressing or enhancing target gene expression. In this sense, this work aimed to evaluate theRNAm expression of NR4A1 and 2, NFKB1 (Nuclear Factor Kappa B Subunit 1) and its inhibitors,NFKBIA and B, in Peripheral Blood Mononuclear Cells from TB patients, who were classifiedaccording to the severity of the disease into mild, moderate and severe. Besides, possibleassociations between them and other plasma mediators of the immune-endocrine response werealso analyzed (IL-6, IL-10, IFNϒ, and DHEA). With regards to NR41 RNAm levels, they werediminished in TB patients with respect to Healthy Controls (HCo) (p=0.03), meanwhile, NR4A2transcript levels in severe TB patients were higher than in HCo (p=0.01). On the other hand,NFKBIA and B transcripts were also augmented in TB patients (p