Bilirubin accumulation and CYP mRNA expression in selected brains regions of jaundiced Gunn rats pups

SILVIA GAZZIN; JAROSLAV ZELENKA; LUCIE ZDRAHALOVA; RENATA KONICKOVA; CARLOS CODA ZABETTA; PABLO J. GIRAUDI; ANDREA L. BERENGENO; ALAN RASENI; MARIA C. ROBERT; LIBOR VITEK; CLAUDIO TIRIBELLI

PEDIATRIC RESEARCH

INT PEDIATRIC RESEARCH FOUNDATION, INC

Lugar: The Woodlands, Texas; Año: 2012 vol. 71

0031-3998

ABSTRACTBackground: few data exist on regional brain bilirubin content in the neonatal period when acute blirubin-induced neurologic damage may occur, and no information is available on regional brain expression of cytochrome P450 monooxygenases (Cyps) that oxidize bilirubin. Methods: bilirubin content was analyzed by HPLC and Cyp1a1, 1a2 and 2a3 mRNA expression by quantitative polymerase chain reaction in cortex (Cx); cerebellum (Cll); superior and inferior colliculi (SC and IC) of 17 days old hyperbilirubinemic (jj) Gunn rats pups from plasma albumin. Results: there was no difference in bilirubin content among brain regions in untreated rats. After i.p. sulphadimethoxine, bilirubin content peaked 4 fold in Cx and SC at 1 h; but 11-13 times in Cll and IC at 24 h; returning to control levesl at 72 h. The Cyp mRNA peaked at 30-70X control at 1 h in Cx and SC, but at 3-9X times control at 24 h in Cll and IC.Conclusions: the close relationship in distinct brain regions between the extent of bilirubin accumulation and induction of mRNA of Cyps suggests they may have a role in protecting selected brain areas from bilirubin neurotoxicity.