PKC alpha activation by TSH plays a key role in thyroid cancer cell proliferation

ROSEMBLIT C ; DIAZ FLAQUE MC; DIAZ ALBUJA JA; BARREIRO ARCOS ML; CAYROL MF; PERONA M; JUVENAL GJ; CREMASCHI GA

Buenos Aires

Congreso; XVII Latin American Thyroid Society Congress; 2019

Latin American Thyroid Society

Introduction Thyroid cancer (TC) incidence has increased significantly within last decades. Thyroid stimulating hormone (TSH) controls thyroid function by binding to TSH G-protein-coupled receptor (TSHR). PKC, a serine/threonine protein kinase, has been widely implicated in malignant transformation, cell survival, motility and invasion. Classical and novel PKC are activated by PLC activation vía tyrosine kinases and G-protein-coupled receptors. Numerous studies established that PKC is overexpressed in cancer and its expression correlates with aggressiveness. However, the role of PKC in TC remains poorly studied. Objectives To study the role of PKCα in TSH-induced proliferation in TC cells.Methods TPC-1 papillary TC cell viability was evaluated by cell titter blue assay. Protein modulation was measured by qPCR, western blot and flow cytometry. siRNA transfection was used to knock down PKCα expression. Results PKC expression in human TC cell lines revealed high protein and mRNA levels relative to normal cell lines. Treatment of TC cells with TSH increased cellular proliferation compared to untreated cells (p