Thyroid hormones induces chemosensitivity to doxorubicin in Jurkat cells through the modulation of enzymes involved in chemotherapy drug metabolism.

DÍAZ FLAQUÉ MC; CAYROL MF; STERLE H; ROSEMBLIT C; PAULAZO A; DÍAZ ALBUJA J; CREMASCHI GA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigaciones Clínicas

THYROID HORMONES INDUCES CHEMOSENSITIVITYTO DOXORUBICIN IN JURKAT CELLS THROUGTHTHE MODULATION OF ENZYMES INVOLVED IN CHEMOTHERAPYDRUG METABOLISMMaría Celeste Díaz Flaqué, María Florencia Cayrol, HelenaSterle, Cinthia Rosemblit, Alejandra Paulazo, Johanna DíazAlbuja, Alicia Klecha, Maria Laura Barreiro Arcos, GracielaCremaschiBIOMED UCA-CONICETThyroid hormones (TH) ? 3,3´,5-triiodo-L-thyronine (T3) and L-thyroxine(T4) ? are important regulators of the metabolism and physiologyof most normal tissues. We recently showed that TH stimulatethe proliferation and metabolism of T cells.To assess the effect of TH on the response to conventional chemotherapy,we determined the Doxorubicin (DOX) dose that inhibitsproliferation by 50% (IC50) in presence or absence of TH. As expected,DOX induces cytotoxicity in a dose-dependent manner in cellstreated or not with TH. It is important to note that under proliferativeconditions, pretreatment with TH sensitizes Jurkat cells to DOXtreatment. Noteworthy pretreatment with TH significantly decreaseschemosensitivity to DOX by 50% (p