Potential of superparamagnetic iron oxide nanoparticles (SPIONs) as radiosensitizer on cancer thyroid cells

PERONA, MARINA; GRISSI, C.; NIEVAS SI; GABET MARIANA; THOMASZ, LISA; OGLIO R; RODRIGUEZ, CARLA; CINTHIA ROSEMBLIT; CREMASCHI GA; DAGROSA, M.A.; HEBE DURAN; JUVENAL GJ; IBAÑEZ IL

Mar del Plata

Congreso; Reunión Anual de Biociencias SAIC, SAB, AACYTAL; 2023

SAIC, SAB, AACYTAL

Thyroid carcinomas are generally well-behaved malignancies thatrespond to standard treatments. However, a subset of thyroid carcinomas of follicular epithelial origin (well-differentiated, WD; poorlydifferentiated, PD; or anaplastic, A) is highly aggressive. Externalbeam radiotherapy could be a treatment option for high risk patients. Superparamagnetic iron oxide nanoparticles (SPIONs) havebeen used in cancer diagnosis and therapy. SPIONs can increasereactive oxygen species (ROS). This characteristic could be combined with radiotherapy to optimize the clinical outcome. The aimwas to study the radiosensitizing properties of SPIONs in thyroidcells. Methods: SPIONs were synthesized and stabilized by methyl- poly(ethylene glycol) (mPEG). Thyroid cancer (WD: TPC-1, PD:WRO and A: 8505c) cells were incubated with different concentrations of mPEG-coated SPIONs. Cell viability was measured byMTT method. Intracellular SPIONs content by measuring the Feconcentration per cell was performed by ICP-AES at 2, 4 and 24hours. Intracellular ROS levels using the fluorescent dye 2’, 7’- dichlorofluorescein-diacetate (DCFH-DA) and survival fraction at 2 Gy(SF2) were measured. Results: 24 hours incubation with SPIONsdid not affect cell viability (0- 100 µg/ml). Intracellular iron contentsignificantly increased at 2, 4 and 24 hours in cells incubated withSPIONs (p