HO-1 genetic variants and its effects in thyroid cancer biology

LUCÍA FERNANDEZ CHAVEZ; GEORGINA PAMELA COLO; ALONSO ELIANA NOELIA; FERRONATO MARIA JULIA; FERMENTO MARIA EUGENIA; ROSEMBLIT C; ALEJANDRO CARLOS CURINO; MARIA MARTA FACCHINETTI

Mar del Plata

Congreso; Reunión Anual de Biociencias SAIC, SAB, AACYTAL; 2023

SAIC, SAB, AACYTAL

In previous studies on human thyroid cancer (TC), we observedelevated levels of hemeoxygenase-1 (HO-1) protein in both cytoplasmic and nuclear compartments. Additionally, increased HO-1mRNA expression correlated with tumor progression. Activationof HO-1 through hemin treatment in the TPC-1 cell line promotedcell viability, proliferation, migration, and cell cycle progression,while inhibition via ZnPP had opposite effects. This study aimed toinvestigate the impact of genetically overexpressed HO-1 variants(full-length - FL, enzymatically inactive - H25A, and nuclear truncated - t-HO1) on cancer-related processes. Using stable transfections of these variants into TPC-1 cells, we observed that FL andH25A forms were predominantly overexpressed in the cytoplasm,while t-HO-1 accumulated in the nucleus. Overexpression of FL andt-HO-1 significantly enhanced cell viability (p