Molecular pathology of acute kidney injury in a choline-deficient model and fish oil protective effect

DENNINGHOFF, VALERIA; OSSANI, GEORGINA; UCEDA, ANA; RUGNONE, MATIAS; FERNÁNDEZ, ELMER; FRESNO, CRISTÓBAL; GONZÁLEZ, GERMAN; DÍAZ, MARIA LUISA; AVAGNINA, ALEJANDRA; ELSNER, BORIS; MONSERRAT, ALBERTO

EUROPEAN JOURNAL OF NUTRITION

DR DIETRICH STEINKOPFF VERLAG

Año: 2014 vol. 53 p. 897 - 906

1436-6207

Purpose: The aim of this work was to investigate the potential protective effects of fish oil on the basis of kidney transcriptomic data on a nutritional experimental model. Methods: Male weanling Wistar rats were divided into four groups and fed choline-deficient (CD) and choline-supplemented (CS) diets with vegetable oil (VO) and menhaden oil (MO): CSVO, CDVO, CSMO and CDMO. Animals were killed after receiving the diets for 6 days. Total RNA was purified from the right kidney and hybridized to Affymetrix GeneChip Rat Gene 1.0 ST Array. Differentially expressed genes were analyzed. Results: All CSVO, CSMO and CDMO rats showed no renal alterations, while all CDVO rats showed renal cortical necrosis. A thorough analysis of the differential expression between groups CSMO and CDMO was carried out. There were no differential genes for p < 0.01. The analysis of the differential expression between groups CSVO and CSMO revealed 32 genes, 11 were over-expressed and 21 were under-expressed in CSMO rats. Conclusions: This work was part of a large set of experiments and was used in a hypothesis-generating manner. The comprehensive analysis of genetic expression allowed confirming that menhaden oil has a protective effect on this nutritional experimental model and identifying 32 genes that could be responsible for that protection, including Gstp1. These results reveal that gene changes could play a role in renal injury. © 2013 Springer-Verlag Berlin Heidelberg.