Influence of culture medium supplementation with linoleic acid on survival to cryopreservation of in vitro bovine embryos] [Influencia de la suplementación del medio de cultivo con ácido linoleico en la supervivencia a la congelación de embriones bovinos

BALLESTEROS B; TRIBULO H; BO G; MUTTO A

Revista electronica de veterinaria

Veterinaria Organizacion

Lugar: Cordoba; Año: 2017 vol. 18 p. 12 - 17

1695-7504

Studies report that the addition of linoleic acid to bovine embryo production media in vitro improves its resistance to conventional freezing. In this study the effect on survival of in vitro bovine embryos under freezing was evaluated by the addition of linoleic acid (LA) in the culture medium on days 3 or 5. A total of 1452 oocytes were recovered and assigned to 4 experimental groups and a control group. Six replicates were made to obtain approximately 100 freeze-Thawed embryos per group. Group 1: 100 ?M on Day 3, group 2: 50 ?M on Day 3, group 3: 100 M on Day 5, group 4: 50 ?M on Day 5. The embryos were frozen and one week after they were thawed to evaluate the percentages of re-expansion (24 h) and hatching (72 h). The results were analyzed by ANOVA and when the differences were significant, the means between the groups were compared by Fischer´s LSD Test (p